Defination of serum osmolality test

  • Definition of Osmolality:
  1. A more exact measurement of urine concentration than specific gravity (SG), Depends on the number of solute particles in a unit of solution.
  • Clinical Importance:
  1. Provides more information on renal function when both serum and urine osmolality are tested together. The normal urine-to-serum osmolality ratio is 3:1.
  2. A high ratio indicates concentrated urine and a low ratio suggests poor kidney concentrating ability

Specimen :

Urine, Spot for 24 hours without preservatives. Samples are stable for 7 days at 15-250°C, 7 days at 2-8°C; andstable for long time at 00°C.

Serum Osmolality Test Calculator

About the Author

Clinical Laboratory Scientist, Lab Manager, Clinical Microbiologist

Related Caculator  

Normal serum osmolality Adults: 280–303 mOsm/kg H₂O 2O (280–303 mmol/kg H 2O)

Newborns: as low as 266 mOsm/kg H 2O (266 mmol/kg H 2O)

Serum Osmolality Calculator

Serum Osmolality Calculator

Formula: Osm = (Na × 2) + (Glucose ÷ 18) + (BUN ÷ 2.8)

Result

Calculated Serum Osmolality:

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Interpretation

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Note: This calculator provides a calculated serum osmolality. Clinical labs may report a measured osmolality; differences (osmolal gap) can occur, especially with substances like ethanol, methanol, ethylene glycol, or mannitol.

Reference Values

Urine (mOsm/kg H2O):

  • 24-hour specimen: 300–900 mOsm/kg H2O
  • Random specimen: 50–1200 mOsm/kg H2O

Serum (mOsm/kg H2O):

  • Adults: 280–303 mOsm/kg H2O (≈ 280–303 mmol/kg H2O)
  • Newborns: as low as 266 mOsm/kg H2O (≈ 266 mmol/kg H2O)
serum osmolality test

Interfering factors

  1. Decreases in osmolal gap are associated with altitude, diurnal variation with water retention at night, and
    some drugs.
  2. Some drugs also cause increases in osmolal gap.
  3. Hypertriglyceridemia and hyperproteinemia cause an elevated osmolal gap.
  4. Radiographic contrast medium within 3 days
  5. Intravenous sodium administration
  6. Intravenous dextrose and water administration