Genomic DNA Library Quantification Calculator: From Concentration to Molarity
What is a Genomic DNA Library?
A genomic DNA library is a carefully organized collection of DNA fragments that together represent the entire genome of an organism. Think of it as a genetic “archive” — every single piece of DNA is stored in smaller, manageable units called clones. These fragments are inserted into vectors (plasmids, cosmids, or bacteriophages) and maintained in host cells, often E. coli.
About the Author
Clinical Laboratory Scientist, Lab Manager, Clinical Microbiologist
Genomic DNA Library Quantification Calculator
Calculate molarity from Qubit dsDNA HS Assay measurements
Result
Calculation Explanation
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Note: This calculator uses the formula: Molarity (nM) = (Concentration × 10⁶) / (660 × Library Size). The library size is typically 400 bp for genomic DNA libraries.
Common Applications:
• Quantifying genomic DNA libraries for NGS
• Normalizing library concentrations for sequencing
• Calculating molarity from Qubit dsDNA HS Assay measurements
